Coding

Part:BBa_K4877011

Designed by: Team iGEM Bielefeld-CeBiTec   Group: iGEM23_Bielefeld-CeBiTec   (2023-10-12)


T2A self cleaving peptide

The T2A self cleaving peptide induces a cleavage of the nascent peptide during ribosomal translation, which causes the peptides upstream and downstream of the T2A sequence to be released as distinct peptides [1]. It can be used to create multi-cistronic open reading frames.

[1]Wang X, Marchisio MA. Synthetic polycistronic sequences in eukaryotes. Synth Syst Biotechnol. 2021 Sep 15;6(4):254-261. doi: 10.1016/j.synbio.2021.09.003. PMID: 34584993; PMCID: PMC8449083.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None